What is rnase p

Finally, it has been shown that transcription of a human U6 snRNA gene could be reconstituted by the use of recombinant core transcription factors and purified Pol III Apparently, RNase P is not required for transcription in this experimental system in which naked DNA is accessible to transcription factors.

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Biochemistry 43 , — Influence of metal ions on the ribonuclease P reaction. Distinguishing substrate binding from catalysis. Brannvall, M. Metal ion cooperativity in ribozyme cleavage of RNA. USA 98 , — Cuzic, S. Nucleic Acids Res. Kikovska, E. Biochemistry 35 , — Pan, T. Higher order folding and domain analysis of the ribozyme from Bacillus subtilis ribonuclease P. Biochemistry 34 , — Fang, X. Nature Struct. Kent, O. Kinetic analysis of the M1 RNA folding pathway. Warnecke, J.

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First sequence-comparative RNA-structure analysis based on genes from complex natural microbial communities. Evolutionary perspective on the structure and function of ribonuclease P, a ribozyme. Haas, E. Siegel, R. Mycoplasma fermentans simplifies our view of the catalytic core of ribonuclease P RNA. RNA 2 , — Use of photoaffinity crosslinking and molecular modeling to analyze the global architecture of ribonuclease P RNA.

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RNA tertiary structure mediation by adenosine platforms. Klosterman, P. RNA 9 , — Hardt, W. Heide, C. RNA 5 , — Multiplex PCR is the use of more than one probe and primer pair in the same tube. These guidelines are simple and easy to follow. Many traditional variables, such as magnesium chloride concentration and the thermal cycling protocol itself, have been standardized, greatly reducing assay development time.

Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes with different reporter dyes are combined in a single tube.

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